Invitrogen A-21202

Invitrogen A-21202

Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488

產(chǎn)品信息

應(yīng)用

建議稀釋比

免疫組化 (IHC)

1-10 µg/mL

免疫細(xì)胞化學(xué) (ICC/IF)

0.2 µg/mL

產(chǎn)品規(guī)格

種屬反應(yīng)

Mouse

宿主/亞型

Donkey / IgG

分類

Polyclonal

類型

Secondary Antibody

抗原

Gamma Immunoglobins Heavy and Light chains

偶聯(lián)物

Alexa Fluor™ 488查看其它偶聯(lián)染料 

激發(fā)/發(fā)射光譜

499/520 nm查看光譜spectra

形式

Liquid

濃度

2 mg/mL

純化類型

purified

保存液

PBS, pH 7.5

內(nèi)含物

5mM sodium azide

保存條件

4° C, store in dark

運輸條件

Ambient

RRID

AB_141607

靶標(biāo)

IgG

交叉吸附

Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum

抗體形式

Whole Antibody

產(chǎn)品詳細(xì)信息

To minimize cross-reactivity, these donkey anti-mouse IgG whole antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.

Product will be shipped at Room Temperature.

靶標(biāo)信息

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.